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Optical Data for TFAX 488, SE Emission Color 2 days ago Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1 cm-1; quantum yield = 0.92. Optical Data for TFAX 488, TFP Emission Color source of excitation is the 488 nm line of the Argon-Ion laser. For details of coupling see our recommended labeling procedure at www.atto-tec.com - Support - Downloads - General Procedures. Product Information: ATTO 488 300 400 500 600 700 800 900 1000 wavelength, nm absorption fluorescence l abs = 500 nm e max = 9.0 x 104 M-1 cm-1 l fl = 520 nm h fl = 80 % t fl Extinction Coefficient (ε) (M-1cm ) Fluorescence Quantum Yield (φ) Fluorescence Life Time (τ) (ns) HiLyte Fluor™ 405 404 428 35,000 0.54 Not available HiLyte Fluor™ 488 497 525 70,000 0.91 4.1 HiLyte Fluor™ 555 550 566 150,000 0.10 0.3 HiLyte Fluor™ 594 593 616 80,000 0.90 4.2 HiLyte Fluor™ 647 649 672 250,000 0.33 1.0 AF 532 NHS AF532: Aldehyde C2 Aldehyde: Alexa Fluor 405 NHS AF405: Alexa Fluor 488 C5 Maleimide AF 488M: Alexa Fluor 488 NHS AF 488 Extinction Coefficient (ε) 75000 M-1 cm-1: Quantum Yield (φ) 0.92: Closest Laser line: 488 nm: Reactive Group: Isothiocyanate: Reactivity: Primary amines: Cell Permeable: Yes Extinction coefficients: AF488: ε dye = 73,000 cm-1 M-1 dsDNA: ε base = 6,600 cm-1 M-1 ssDNA: ε base = 8,900 cm-1 M-1 oligonucleotide: εbase = 10,000 cm-1 M-1 Example: A dye to base ratio of 0.05 corresponds to an incorporation of 10 dye-dUTP nucleotides into a DNA fragment containing 200 nucleotides or, respectively, into a 100 bp PCR Extinction coefficient: 155,000 cm-1M-1 Spectrally similar dyes: tetramethylrhodamine Molecular weight: ~1250 Typical Conjugation Reaction You can conjugate amine-reactive reagents with virtually any protein or peptide (the provided protocol is optimized for IgG antibodies). 4. Extinction coefficient behaviour with wavelength. The aim of this work is to study the atmospheric extinction coefficients at several wavelengths in different atmospheric conditions (clear and dusty days) over the Canarian observatories.

2019-06-01 Summary In case of fire, the visibility plays a major role as it limits the occupants’ orientation capabilities and the perception of signs. These effects are determined by the light extinction due and extinction coefficient and L the pathlength of the 1-cm cuvette, (25, 28). And it was 31.9mg/L. From the stock solution, 6 aliquots were taken to obtain six different concentrations: 1.595, 3.190, 5.582, 6.380, 7.975, and 12.760 mg/L.

For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Yes, you can use the extinction coefficient of eGFP at 488nm.

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The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets. molar decadic extinction coefficient at the longest-wavelength absorption maximum l fl fluorescence maximum h fl fluorescence quantum yield t fl fluorescence decay time CF 260 CF 260 = e 260 /e max. Correction factor used in calculation of degree of labeling (DOL) in case of dye-DNA conjugates CF 280 CF 280 = e 280 /e max. Correction factor used in calculation of The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name.

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5 Apr 2005 bance of the protein (protein extinction coefficients determined by various means) at 280 nm and the absor- bance of Alexa-Fluor 488 (molar Extinction Coefficient [Alexa Fluor 488]. The molar extinction coefficient (ε) for Alexa Fluor 488 is 73,000 cm-1M 22 May 2017 For example, DyLightTM 350 has an extinction coefficient value of 15,000 (dim) As an example, FITC cannot be used with Alexa Fluor® 488. proportional to the product of the extinction coefficient and the quantum yield. Note that the 488-nanometer argon-ion laser spectral line excites Alexa Fluor Extinction coefficient: 70,000. Molecular weight: ~914. Excitation laser line: 488 nm.

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1329 ± 1021. 244 ± 302. 1.16 ± 0.61 p. 3096 ± 3122.

1.24 ± 0.66 r. 1329 ± 1021. 244 ± 302. 1.16 ± 0.61 p.
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Alexa Alexa Fluor 488 N-hydroxysuccinimidyl ester (AF488 NHS-ester) is an 488 ( AF488), is optimally excited with a 488 nm laser line (extinction coefficient of Structural formula of AF488 Maleimide (also known as Alexa Fluor® 488) Structural formula of AF488 Maleimide.

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Extinction. Coefficient (ε). (M-1cm-1).